ബാക്ടീരിയൽ വാട്ടത്തിൻ്റെ ജൈവനിയന്ത്രണത്തിനായി സോളനേഷ്യസ് വിളകളുടെ റൈസോസ്ഫിയറുകളിൽ നിന്ന് ഡിഎപിജി ഉൽപ്പാദിപ്പിക്കുന്ന ഫ്ലൂറസെൻ്റ് സ്യൂഡോമോനാഡുകളുടെ വിരുദ്ധ പ്രവർത്തനത്തിൻ്റെ വിലയിരുത്തൽ

Pseudomonads produce a wide array of antimicrobial compounds including 2,4-diacetylphloroglucinol (DAPG), which possess a broad-spectrum antagonistic effect against phytopathogens. Rhizospheric soil samples were collected from the Solanaceous crops for isolation of fluorescent pseudomonads (FPs). Out of the 32 fluorescent bacterial isolates, 21 showed antagonistic activity against റാൽസ്റ്റോണിയ സോളനേസറം (NAIMCC-B-01630) with the highest zone of inhibition at 38.33 mm. Among them, 11 bacterial isolates depicted the production of DAPG in Kings’ B medium. DAPG production was detected by TLC and quantified through HPLC analysis. Moreover, the bacterial isolate OD13 exhibited higher DAPG production. Further, the presence of the DAPG-encoding phlD gene within the size of 629 bp and 725 bp was also validated through amplification. These bacterial isolates belonged to the genera സുഡോമാസസ് as confirmed by morpho-physiological characterization using the VITEK 2 system. The higher DAPG-producing bacterial isolate was identified as സുഡോമാനോസ് ഏറുഗ്നോനോ by polyphasic approach with GenBank accession number OQ781265. Thus, the DAPG-producing rhizospheric സുഡോമാസസ് strains can be used as biocontrol agents for bacterial wilt. However, field study is highly essential before its successful application.

ഫോട്ടോ: EPPO (2024) EPPO ഗ്ലോബൽ ഡാറ്റാബേസ്. https://gd.eppo.int

Reference: Suansia, A., Senapati, A. K., Samantaray, D., & Mohanty, M. K. (2024). Evaluation of antagonistic activity of DAPG-producing fluorescent pseudomonads from rhizospheres of Solanaceous crops for biocontrol of bacterial wilt. ജേണൽ ഓഫ് ഫൈറ്റോപത്തോളജി, 172, e13315. https://doi.org/10.1111/jph.13315